Numera®

Advanced Bioprocess Sampling Solution

Numera® is a fully automated sampling system that never sleeps, integrating and improving every process analytical technology with reliable real-time results 24/7. Combined with Lucullus® software, Numera® is a seamless all-in-one sampling solution.

Sampling from up to 16 vessels

2 to 3 ml sampling volume

Sampling time ~8 minutes

Sample storage and cooling

Sample transfer to up to 4 analyzers

Unique filtration technology for cell removal

Automated sampling 24/7

High frequency and low volume sampling

Individual sample preparation including consistent cell removal.

On-line measurements with common analyzers

Automated data import from analyzers and advanced feedback control loops with Lucullus®

About Numera®

Numera® is a modular PAT system. Each installation includes a Control Module, a Routing Module, and at least one Multiplexer Module, while the Dilution and Filtration Modules for sample preparation are optional.

The Autosampler/Sample Collector stores retained samples at 4°C for further processing.

Numera® can be used in many applications – from single-use, bench-top reactors, up to production.

The modular design of Numera® allows configurations for single reactor sampling and storage up to multiple reactor sampling including dilution, filtration, and integration of various on-line analyzers.

Control Module

Touchscreen

Configuration settings for system preparation, cleaning, and settings

Various communication ports

Compressed air distribution
Multiplexer Module

Multiplexing of up to 4 bioreactors per module

Up to 4 modules per Numera® system

Pinch valve sterility barrier with ethanol rinsing

Sterile air blow back mechanism to prevent cross-contamination and preserve low volume sampling
Routing Module

Individual sample distribution within one process

Direct transfer to maximum 4 analyzers (biochemistry analyzers or cell counters)
Dilution Module

Dilution from 1:2 to 1:20

Reagent addition, mixing, and incubation

Precise and reproducible sample processing
Filtration Module

Unique cross-flow filtration technology

Low protein binding PES membrane

Up to 700 filtrations per coil

Membrane pore sizes: 0.22 μm, 0.45 μm, 0.65 μm, and 1.2 μm

Fresh membrane section for every filtration cycle avoiding cross-contamination and clogging

Setup and Configuration

Autosampler / Sample Collector

Storage of retained samples or intermediate storage of samples for further processing is achieved with the Autosampler. In addition, manually taken samples can be stored for processing with the Numera® and/or for transfer to analyzers. Cooling is guaranteed in a range of 4 to 20 °C. There are two different sizes of Autosampler available. Both are equipped with a syringe pump and a rinse station.

The Autosampler can be equipped with 2 well plates per Peltier rack module. Securecell offers well plates for 1.5 ml and 4.0 ml glass vials. Standard deep-well plates with 2, 5, or 10 ml can also be used.

ASX7200

2 Peltier Rack Modules (storage at 4 to 20°C)
4 well plate positions
Optional: 1 HPLC injection valve

ASX7400

4 Peltier Rack Modules (storage at 4 to 20 °C)
8 well plate positions
Optional: 1 or 2 HPLC injection valve(s)

ACCESSORIES

Well plates

40 x 1.5 ml vials
24 x 4.0 ml vials
Standard deep-well plates with 2, 5, or 10 ml

HPLC/UHPLC Injection Valves

Variants from 5,000 to 20,000 psi
Vertical port injector
Sample loop (ID 0.14 mm) from 5 to 20 μl

Product Configuration

Reactor Connection and Sterility

Reactor Connections

Numera® can be used in many applications – from single use, benchtop reactors, up to production. The appropriate connection options are available for all purposes. The modular concept includes:

• Autoclavable sampling sets
• Sample lines 1 – 10m

Single-Use Bioreactors

+ Sample lines

Benchtop Reactors

+ Sample lines
+ Dip tube
+ Adapters

Stainless Steel Reactors

+ Sample lines
+ Sampling port

Single-Use Sampling Sets

At the Multiplexer, autoclavable sampling sets including all tubings for sterile sampling, ethanol cleaning and flush back with sterile air, are installed for each sample vessel.

Sample Lines

From 1 to 10 meters are used to attach the sampling set at the Multiplexer to the corresponding bioreactor interface with Luer-Lock screw connections.

Dip Tubes

Stainless steel dip tubes with a Luer-Lock connector are used in combination with a suitable reactor adapter to sample from benchtop (glass) bioreactors.

Adapters

Adapters from 10 – 19 mm and PG13.5 are available to mount the dip tubes to any common headplate port.

Aseptic Sampling Port

The Aseptic Sampling Port enables sampling from stainless steel bioreactors using the 25 mm side port. The connection to the Numera® System is made with a Septum Piercing Needle with side entry. The needle comes with a holder that fits to the Aseptic Sampling Port and with a Luer-Lock connector.

Sampling & Sterility Concept

Analytics and Sample Management

Analyzer Integration

Sample Management with Lucullus Software

“Securecell’s support goes beyond the simple supplier-customer relationship. With Securecell we chose a partner that is actively supporting us in our mission of automating and digitalizing our laboratory environment.”

- Nick Wierckx

Professor at Institute of Bio- and Geosciences Forschungszentrum Jülich

Numera®

Advanced Bioprocess Sampling Solution

About Numera®

Numera® Modules

Control Module

Multiplexer Module

Routing Module

Dilution Module

Filtration Module

Setup and Configuration

Autosampler / Sample Collector

ASX7200

ASX7400

ACCESSORIES

Well plates

HPLC/UHPLC Injection Valves

Product Configuration

Reactor Connection and Sterility

Reactor Connections

Single-Use Bioreactors

Benchtop Reactors

Stainless Steel Reactors

Single-Use Sampling Sets

Sample Lines

Dip Tubes

Adapters

Aseptic Sampling Port

Sampling & Sterility Concept

Analytics and Sample Management

Analyzer Integration

HPLC Integration

Sample Management with Lucullus Software

- Nick Wierckx

Insights

DETERMINATION OF METABOLITES IN STARTER CULTURE DEVELOPMENT – THE PAST AND THE PRESENT

ENHANCED AAV2 PRODUCTION PROCESS CONTROL AND INSIGHT THROUGH INTEGRATION OF NUMERA® AUTOMATED SAMPLING AT CGT CATAPULT

VIRTUAL TOUR OF THE DIGITIZED BIOPROCESSING LABORATORY AT THE ZHAW

Unique, modular sampling system for bioprocesses

Related Products and Technologies

Lucullus®

SampleBus

Integra1 64