Numera® 3 provides seamless integration to your lab automation with simpler tube management, a larger touchscreen, and refreshed UI. Whether you’re working with single-use bench-top reactors or scaling up to production, Numera® 3 adapts to your needs.
The Numera® 3 Sample Collector stores retained samples for further processing. The samples can be cooled to 4 °C. The Sample Collectors are available in two different configurations: the smaller ASX-7200 and the larger ASX-7400. Both models are equipped with a syringe pump and a rinse station.
Different sample racks can be chosen depending on customer needs. Securecell offers vial racks for 1.5 ml, 4.0 ml, and 10 ml glass vials. Standard deep-well plates with 2, 5, or 10 ml can also be used.
Sample Collection and Storage
Automatically collect samples from connected bioreactors and preserve them at 4–20°C to ensure sample integrity for subsequent analysis.
Automated Sample Processing
Automate sample processing with dilution (up to 1:100) and advanced filtration, enabling reliable collection of cell-free samples.
Integrated On-line Analytics
Achieve real-time insights through automated, parallel analysis of your samples with integrated cell counters, biochemistry analyzers, and LC systems.
+ Sample lines
+ Ambr® Interface
+ Sample lines
At the Multiplexer, autoclavable sampling sets including all tubings for sterile sampling, ethanol cleaning, and blow-back with sterile air, are installed for each sample vessel.
From 1 to 10 meters are used to attach the sampling set at the Multiplexer to the corresponding bioreactor interface with Luer-Lock screw connections.
Stainless steel dip tubes (30 cm and 50 cm) with a Luer-Lock connector are used in combination with a suitable reactor adapter to sample from benchtop bioreactors.
From 10 mm, 11 mm, 12 mm, and PG13.5 are available to mount the dip tubes to any common headplate port.
The Aseptic Sampling Port enables sampling from stainless steel bioreactors using the 25 mm side port. The connection to the Numera® System is made with a Septum Piercing Needle with side entry. The needle comes with a holder that fits to the Aseptic Sampling Port and with a Luer-Lock connector.
Proven Sterility and Reliable Contamination
Control
The Numera® Sampling System has been rigorously validated to ensure uncompromising sterility and protection against cross-contamination—even under challenging conditions.
Proof of sterility in a technical run
Validation of Tubing Sterilization
To confirm sterility, sampling tubes were intentionally contaminated with Bacillus stearothermophilus, one of the most resistant microorganisms used in sterility testing, and after autoclaving no viable organisms were detected, demonstrating effective sterilization.
Long-Term Sterility During Sampling
To assess sterility maintenance during extended use, the system was connected to a 3.6 L bioreactor containing sterile culture medium. Over 15 days, samples were taken every 2 hours— more than 180 individual sampling events. Throughout the trial, no turbidity, pH shifts, oroxygen level changes were observed (see figure). This confirms that the pinch valves ensure a sterile barrier from contamination, even during continuous, high-frequency sampling.
Cross-Contamination Testing
The system was further challenged with alternating samples from sterile and deliberately contaminated media over 10 days, totaling 1,000 samples, and despite repeated switching no contamination was transferred between sterile and non-sterile vessels, with sterility verified visually, spectrophotometrically, and by streak plating.
With proven sterility, long-term reliability, and robust protection against cross-contamination, the Numera® Sampling System provides confidence in every sample—ensuring accurate results and safeguarding your processes.
The Autosampler setup can include an injection valve for HPLC (up to 20,000 psi). Direct injection provides loss-free and fast analytics using HPLC systems from various vendors.
Numera® 3 can be integrated with a variety of analyzers to perform on-line, at-line, and offline analysis. These analyzers include:
Securecell provides the specific accessories and instructions required for the integration of each analyzer.
The combination of the automated sampling system, Numera®, and the Lucullus® Process Information Management System, enables the orchestration of the whole workflow comprising sampling, measurement, process monitoring, and control.
