Introduction
Can you find the right operating conditions for your shear-sensitive cell culture before performing costly experiments?In this webinar, we explore how Digital Bioreactors can enable faster and more predictable scale-up. The underlying technology uses advanced CFD simulations, providing a first-principles basis for predicting bioreactor performance. However, building a reliable Digital Bioreactor requires experimental validation. We will show how a range of experiments supported the development of a Digital Bioreactor of a 250 mL stirred-tank bioreactor. These included microcarrier suspension experiments, 4D particle tracking velocimetry (4D-PTV), and automated kLa measurements using Lucullus.
Once validated, the Digital Bioreactor serves as a virtual testbed that predicts how operating conditions affect suspension, mass transfer, and hydrodynamic stress. Rather than relying solely on trial-and-error experiments, we can screen operating conditions in-silico and identify promising experimental conditions, reducing the number of costly experiments needed during process development. Our principles can be applied to larger scales, supporting faster scale-up towards (clinical trial) production volumes. To demonstrate its practical value, we will show how the Digital Bioreactor guided the selection of operating conditions for iPSC-derived embryoid body formation, a process being developed for cRBC production.
What You Will Learn
During the webinar:
- Why conventional scale-up criteria fall short for shear-sensitive cultures
- How a Digital Bioreactor is built and validated experimentally
- How in-silico screening helps find optimal operating conditions across scales
This webinar meets twice; please join based on your availability.
Tue, December 8, 2026, 09:00 - 10:00 AM CET
Tue, December 8, 2026, 05:00 - 06:00 PM CET
Format: Online webinar
Participation: Free of charge
Duration: 50 min (content) + 10 min (Q&A)
Collaboration with
